Research Papers

Utilisation of semiconductor sequencing for the detection of predictive biomarkers in glioblastoma
Published: March 25, 2022 DOI: https://doi.org/10.1371/journal.pone.0245817
Gareth H Williams, Alexander Llewelyn, Robert Thatcher, Keeda-Marie Hardisty, Marco Loddo

SARS-CoV-2 testing and sequencing for international arrivals reveals significant cross border transmission of high risk variants into the United Kingdom
EClinical Medicine by the Lancet Published:July 14, 2021 DOI: https://doi.org/10.1016/j.eclinm.2021.101021
Gareth H Williams, Alexander Llewelyn, Ruben Brandao, Kaiya Chowdhary,Keeda-Marie Hardisty, Marco Loddo (2021)

Clinical, Radiometabolic and Immunologic Effects of Olaparib in Locally Advanced Triple Negative Breast Cancer: The OLTRE Window of Opportunity Trial
Front Oncol. 2021; 11: 686776. Published online 2021 Jun 28. doi: https://doi.org/10.3389/fonc.2021.686776
Alfredo Molteni, Alice Bruson, Carla Strina, Carlo Azzini, Daniele Generali, Davide Cerra, Elena Marasco, Elisa Zago, Fabiola Giudici, Francesco Schettini, Gareth H Williams, Giovanni Scambia, Giuseppina Barbieri, Giuseppina Ferrero, Ida Paris, Luciano Xumerle, Manuela Milani, Marco Loddo, Marco Ungari, Maria Chiara Lazzari, Maria Rosa Cappelletti, Marianna Sirico, Nicoletta Ziglioli, Ottavia Bernocchi, Sergio Aguggini, Silvia Paola Corona, Valeria Cervoni

The actionable genomic mutational landscape in solid tumours
J Clin Oncol 38: 2020 (suppl; abstr e13642) DOI: https://doi.org/10.1200/JCO.2020.38.15_suppl.e13642
Loddo M, Hardisty KM, Thatcher RP, Haddow TE, Williams GH (2020)

Immunofocus-PD-L1 high throughput quantitative next generation sequencing assay
J Clin Oncol 38: 2020 (suppl; abstr e13521) DOI: https://doi.org/10.1200/JCO.2020.38.15_suppl.e13521
Williams GH, Thatcher RP, Haddow TE, Hardisty KM, Loddo M (2020)

Druggable fusion gene landscape in solid tumors
J Clin Oncol 38: 2020 (suppl; abstr e13517) DOI: https://doi.org/10.1200/JCO.2020.38.15_suppl.e13517
Williams GH, Thatcher RP, Nevado B, Haddow TE, Hardisty KM, Loddo M (2020)

Inter-observer Reliability of Programmed Cell Death Ligand-1 Scoring Using the VENTANA PD-L1 (SP263) Assay in Non-Small Cell Lung Cancer
J Thorac Oncol. 2019 Nov 25. pii: S1556-0864(19)33664-0. doi: https://doi.org/10.1016/j.jtho.2019.11.010.
Williams GH, Nicholson AG, Snead DRJ, Thunnissen E, Lantuejoul S, Cane P, Kerr KM, Loddo M, Scott MLJ, Scorer PW, Barker C. (2019)

Assessment of PD-L1 mRNA and protein expression in non-small cell lung cancer, head and neck squamous cell carcinoma and urothelial carcinoma tissue specimens using RNAScope and immunohistochemistry.
David Jonathan Duncan ,Marietta Scott,Paul Scorer et al ()

Mapping the binding sites of antibodies utilized in programmed cell death ligand-1 predictive immunohistochemical assays for use with immuno-oncology therapies
Mod Pathol. 2019 Sep 26. doi: https://doi.org/10.1038/s41379-019-0372-z. [Epub ahead of print] Erratum in: Mod Pathol. 2019 Oct
Lawson NL, Dix CI, Scorer PW, Stubbs CJ, Wong E, Hutchinson L, McCall EJ, Schimpl M, DeVries E, Walker J, Williams GH, Hunt J, Barker C. (2019)

Immunophenotypic analysis of cell cycle status in acute myeloid leukaemia: relationship to cytogenetics, genotype and clinical outcome
Br J Haematol. 2018 May;181(4):486-494. doi: https://doi.org/10.1111/bjh.15211. Epub 2018 Apr 20.
Sellar RS, Gale RE, Khwaja A, Garbowski M, Loddo M, Stoeber K, Williams GH, Linch DC

DNA replication licensing factor MCM2, geminin, and Ki67 define proliferative state and are linked with survival in oral squamous cell carcinoma
Eur J Oral Sci. 2018 Jun;126(3):186-196. doi: https://doi.org/10.1111/eos.12420.
Al-Hazmi N, Alhazzazi T, Williams G, Stoeber K, Al-Dabbagh R

Consistency of tumor and immune cell programmed cell death ligand-1 expression within and between tumor blocks using the VENTANA SP263 assay
Diagnostic Pathology, (2018) 13:47 DOI: https://doi.org/10.1186/s13000-018-0725-9
Paul Scorer, Marietta Scott, Nicola Lawson et al (2018)

Agreement between Programmed Cell Death Ligand-1 Diagnostic Assays across Multiple Protein Expression Cut-Offs in Non-Small Cell Lung Cancer
Clinical Cancer Research, January 2017; 16-2375 DOI: https://doi.org/10.1158/1078-0432.ccr-16-2375
Marianne J Ratcliffe, Alan Sharpe, Anita Midha et al (2017)

A novel p53-Cdc7 link induced by genotoxic stress
Cell Cycle, DOI: https://doi.org/10.1080/15384101.2017.1304746.
Hisao Masai

Relevance of deep learning to facilitate the diagnosis of HER2 status in breast cancer.
Nature – Scientific Reports, April 2017, 7, 45938 DOI: https://doi.org/10.1038/srep45938
Michel E. Vandenberghe, Marietta L. J. Scott, Paul W. Scorer et al (2017)

Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL
Virchows Arch (2016). doi: https://doi.org/10.1007/s00428-016-2025-7.
Zandra C Deans, Jose Luis Costa, Ian Cree, Els Dequeker, Anders Edsjö, Shirley Henderson, Michael Hummel, Marjolijn JL Ligtenberg, Marco Loddo, Jose Carlos Machado, Antonio Marchetti, Katherine Marquis, Joanne Mason, Nicola Normanno, Etienne Rouleau, Ed Schuuring, Keeda-Marie Snelson, Erik Thunnissen, Bastiaan Tops, Gareth Williams, Han van Krieken, Jacqueline A Hall, On behalf of IQN Path ASBL

A comparative study of PD-L1 diagnostic assays in squamous cell carcinoma of the head and neck (SCCHN)
Annals of Oncology; 27 (suppl_6): 955PD DOI: https://doi.org/10.1093/annonc/mdw376.07
J. Ratcliffe, A. Sharpe, M. Rebelatto et al ()

p53 controls CDC7 levels to reinforce G1 cell cycle arrest upon genotoxic stress
Cell Cycle. 2016 Sep 9:0 DOI: https://doi.org/10.1080/15384101.2016.1231281
Tudzarova S, Mulholland P, Dey A, Stoeber K, Okorokov AL, Williams GH

Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment
Br J Haematol. 2016 Apr 8. doi: https://doi.org/10.1111/bjh.14055
Sellar RS, Fraser L, Khwaja A, Gale RE, Marafioti T, Akarca A, Hubank M, Brooks T, Stoeber K,Williams G, Linch DC

Cdc7 is a potent anti-cancer target in pancreatic cancer due to abrogation of the DNA origin activation checkpoint
Oncotarget. 2016 Feb 23. doi: https://doi.org/10.18632/oncotarget.7611.
Huggett MT, Tudzarova S, Proctor I, Loddo M, Keane MG, Stoeber K, Williams GH, Pereira SP.

Do Cell-Cycle Phase-Specific Markers Predict Disease Grade, Stage, and Outcome in Cervical Carcinoma?
Int J Gynecol Cancer. 2015 Jul;25(6):1066-72.
Kuku S, Proctor I, Loddo M, Kadalayil L, KhoshZaban M, Ledermann J, McCormack M.

Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer
J Pathol. 2014 Aug;233(4):344-56.
Loddo M, Andryszkiewicz J, Rodriguez-Acebes S, Stoeber K, Jones A, Dafou D, Apostolidou S, Wollenschlaeger A, Widschwendter M, Sainsbury R, Tudzarova S, Williams GH.

Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint
J Proteome Res. 2013 Mar 1;12(3):1436-53
Mulvey CM, Tudzarova S, Crawford M, Williams GH, Stoeber K, Godovac-Zimmermann J

Bladder cancer diagnosis and identification of clinically significant disease by combined urinary detection of Mcm5 and nuclear matrix protein 22
PLoS One. 2012;7(7):e40305
Kelly JD, Dudderidge TJ, Wollenschlaeger A, Okoturo O, Burling K, Tulloch F, Halsall I, Prevost T, Prevost AT, Vasconcelos JC, Robson W, Leung HY, Vasdev N, Pickard RS, Williams GH, Stoeber K.

The cell cycle and cancer
J Pathol. 2012 Jan;226(2):352-64.
Williams GH, Stoeber K.

Exploring the interaction between siRNA and the SMoC biomolecule transporters: implications for small molecule-mediated delivery of siRNA
Chem Biol Drug Des. 2012 Jan;79(1):9-21.
Gooding M, Tudzarova S, Worthington RJ, Kingsbury SR, Rebstock AS, Dube H, Simone MI, Visintin C, Lagos D, Quesada JM, Laman H, Boshoff C, Williams GH, Stoeber K, Selwood DL.

Molecular architecture of the DNA replication origin activation checkpoint
EMBO J. 2010 Oct 6;29(19):3381-94.
Tudzarova S, Trotter MW, Wollenschlaeger A, Mulvey C, Godovac-Zimmermann J, Williams GH, Stoeber K.

Two ubiquitin ligases, APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP, sequentially regulate glycolysis during the cell cycle
Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5278-83.
Tudzarova S, Colombo SL, Stoeber K, Carcamo S, Williams GH, Moncada S.

Targeting DNA replication before it starts: Cdc7 as a therapeutic target in p53-mutant breast cancers
Am J Pathol. 2010 Oct;177(4):2034-45.
Rodriguez-Acebes S, Proctor I, Loddo M, Wollenschlaeger A, Rashid M, Falzon M, Prevost AT, Sainsbury R, Stoeber K, Williams GH.

Diagnosis of prostate cancer by detection of minichromosome maintenance 5 protein in urine sediments
Br J Cancer. 2010 Aug 24;103(5):701-7.
Dudderidge TJ, Kelly JD, Wollenschlaeger A, Okoturo O, Prevost T, Robson W, Leung HY, Williams GH, Stoeber K.

Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 IHC and ISH Testing. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics (Book)
First Edition; 2010; Chapter 6; pp.101-122 DOI: https://doi.org/10.1002/9780470875612.ch6
Shan-Rong Shi and Clive R. Taylor (2010)

DNA replication licensing factors and aneuploidy are linked to tumor cell cycle state and clinical outcome in penile carcinoma
Clin Cancer Res. 2009 Dec 1;15(23):7335-44.
Kayes OJ, Loddo M, Patel N, Patel P, Minhas S, Ambler G, Freeman A, Wollenschlaeger A, Ralph DJ, Stoeber K, Williams GH.

Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma
Clin Cancer Res. 2009 Apr 1;15(7):2417-25.
Kulkarni AA, Kingsbury SR, Tudzarova S, Hong HK, Loddo M, Rashid M, Rodriguez-Acebes S, Prevost AT, Ledermann JA, Stoeber K, Williams GH.

Phospho-STAT5 and phospho-Akt expression in chronic myeloproliferative neoplasms
British Journal of Haematology, 2009, Vol. 147: p495-506 DOI: https://doi.org/10.1111/j.1365-2141.2009.07870.x
L. F. Grimwade, L. Happerfield, C. Tristram Lisa Happerfield et al (2009)

Cell-cycle-phase progression analysis identifies unique phenotypes of major prognostic and predictive significance in breast cancer
Br J Cancer. 2009 Mar 24;100(6):959-70.
Loddo M, Kingsbury SR, Rashid M, Proctor I, Holt C, Young J, El-Sheikh S, Falzon M, Eward KL, Prevost T, Sainsbury R, Stoeber K, Williams GH.

Modular assembly using sequential palladium coupling gives easy access to the SMoC class of cellular transporters
Chembiochem. 2008 Jul 21;9(11):1787-96.
Rebstock AS, Visintin C, Leo E, Garcia Posada C, Kingsbury SR, Williams GH, Stoeber K, Selwood DL.

Diagnosis of pancreaticobiliary malignancy by detection of minichromosome maintenance protein 5 in bile aspirates
Br J Cancer. 2008 May 6;98(9):1548-54.
Ayaru L, Stoeber K, Webster GJ, Hatfield AR, Wollenschlaeger A, Okoturo O, Rashid M, Williams G, Pereira SP.

Identification of an arginine-rich motif in human papillomavirus type 1 E1;E4 protein necessary for E4-mediated inhibition of cellular DNA synthesis in vitro and in cells
J Virol 82:9056-9064
Roberts S, Kingsbury SR, Stoeber K, Knight GL Gallimore PH & Williams GH

Cell cycle markers in clinical oncology
Curr Opin Cell Biol. 2007 Dec;19(6):672-9.
Williams GH, Stoeber K.

DNA replication licensing factors and aurora kinases are linked to aneuploidy and clinical outcome in epithelial ovarian carcinoma.
Clin Cancer Res. 2007 Oct 15;13(20):6153-61.
Kulkarni AA, Loddo M, Leo E, Rashid M, Eward KL, Fanshawe TR, Butcher J, Frost A, Ledermann JA, Williams GH, Stoeber K.

Hexameric ring structure of human MCM10 DNA replication factor
EMBO Rep. 2007 Oct;8(10):925-30.
Okorokov AL, Waugh A, Hodgkinson J, Murthy A, Hong HK, Leo E, Sherman MB, Stoeber K, Orlova EV, Williams GH.

Mitogenic growth signalling, DNA replication licensing, and survival are linked in prostate cancer
Br J Cancer. 2007 May 7;96(9):1384-93.
Dudderidge TJ, McCracken SR, Loddo M, Fanshawe TR, Kelly JD, Neal DE, Leung HY, Williams GH, Stoeber K.

DNA replication licensing, and survival are linked in prostate cancer
Br J Cancer. 2007 May 7;96(9):1384-93.
Dudderidge TJ, McCracken SR, Loddo M, Fanshawe TR, Kelly JD, Neal DE, Leung HY, Williams GH, Stoeber K.

Small-molecule mimics of an alpha-helix for efficient transport of proteins into cells
Nat Methods. 2007 Feb;4(2):153-9.
Okuyama M, Laman H, Kingsbury SR, Visintin C, Leo E, Eward KL, Stoeber K, Boshoff C, Williams GH, Selwood DL.

Novel markers of cell kinetics to evaluate progression from cirrhosis to hepatocellular carcinoma
Liver Int. 2006 May;26(4):424-32.
Quaglia A, McStay M, Stoeber K, Loddo M, Caplin M, Fanshawe T, Williams G, Dhillon A.

DNA replication licensing and cell cycle kinetics of normal and neoplastic breast
Br J Cancer. 2005 Nov 28;93(11):1295-300.
Shetty A, Loddo M, Fanshawe T, Prevost AT, Sainsbury R, Williams GH, Stoeber K.

Repression of DNA replication licensing in quiescence is independent of geminin and may define the cell cycle state of progenitor cells
Exp Cell Res. 2005 Sep 10;309(1):56-67.
Kingsbury SR, Loddo M, Fanshawe T, Obermann EC, Prevost AT, Stoeber K, Williams GH.

DNA replication licensing in peripheral B-cell lymphoma
J Pathol. 2005 Feb;205(3):318-28.
Obermann EC, Eward KL, Dogan A, Paul EA, Loddo M, Munson P, Williams GH, Stoeber K.

Mcm2, Geminin, and KI67 define proliferative state and are prognostic markers in renal cell carcinoma
Clin Cancer Res. 2005 Apr 1;11(7):2510-7.
Dudderidge TJ, Stoeber K, Loddo M, Atkinson G, Fanshawe T, Griffiths DF, Williams GH.

DNA replication licensing in somatic and germ cells
J Cell Sci. 2004 Nov 15;117(Pt 24):5875-86.
Eward KL, Obermann EC, Shreeram S, Loddo M, Fanshawe T, Williams C, Jung HI, Prevost AT, Blow JJ, Stoeber K, Williams GH.